Development of a High-Resolution Melting Method for Screening R188H Polymorphism in XRCC2 Gene
نویسندگان
چکیده مقاله:
Background: The High Resolution Melting (HRM) method is a new scanning method for detecting unknown changes in DNA and its advantages have persuaded researchers to recruit it as a screening method. Objectives: Here, we developed a HRM method to screen R188H SNP (rs3218536) of XRCC2 and compared the results with a well known PCR-RFLP technique. Materials and Methods: Genomic DNA samples from 350 healthy individuals were obtained. PCR-HRM analysis and PCR-RFLP method were performed simultaneously. Results: Three different melting profiles corresponding to three different genotypes recognized by HRM analysis. The results of PCR-RFLP showed no discrepancy. Conclusions: We concluded that the HRM technique can be used as a screening method for rapid discrimination of R188H genotypes in XRCC2 gene.
منابع مشابه
development of a high-resolution melting method for screening r188h polymorphism in xrcc2 gene
background: the high resolution melting (hrm) method is a new scanning method for detecting unknown changes in dna and its advantages have persuaded researchers to recruit it as a screening method. objectives: here, we developed a hrm method to screen r188h snp (rs3218536) of xrcc2 and compared the results with a well known pcr-rflp technique. materials and methods: genomic dna samples from 350...
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عنوان ژورنال
دوره 11 شماره 2
صفحات 104- 108
تاریخ انتشار 2013-04-01
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